Use of non-radioactive detection in SSCP, direct DNA sequencing and LOH analysis
نویسندگان
چکیده
منابع مشابه
Use of non-radioactive detection in SSCP, direct DNA sequencing and LOH analysis.
Aims-To develop a protocol that is applicable to single strand conformation polymorphism (SSCP), direct sequencing and loss of heterozygosity analysis of DNA.Methods-The protocol is based on the detection of biotinylated DNA by a Streptavidin-alkaline phosphatase conjugate. Biotinylation of DNA was achieved by using 5'-end biotinylated primers for PCR. After polyacrylamide gel electrophoresis, ...
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ژورنال
عنوان ژورنال: Molecular Pathology
سال: 1996
ISSN: 1366-8714
DOI: 10.1136/mp.49.2.m118